Journal: Journal of the American Heart Association

Article Title: Direct Cardiac Actions of the Sodium Glucose Co‐Transporter 2 Inhibitor Empagliflozin Improve Myocardial Oxidative Phosphorylation and Attenuate Pressure‐Overload Heart Failure

doi: 10.1161/jaha.120.018298

Figure Lengend Snippet: Figure 5. Chronic empagliflozin treatment improved oxidative phosphorylation in failing hearts. (A) to (D) Mean glucose and oleate acid oxidation, glycolysis, and glucose uptake in isolated perfused hearts from mice in different groups at the end of the protocol (n=5–6). (E) Calculated ATP generation in Sham, Sham+empagliflozin, TAC and TAC+empagliflozin groups (n=5–6). (F) The cardiac output of the isolated perfused hearts (n=5–6) in each group. (G) Left: representative blots of CD36, PPARα, and GAPDH. Right: Quantitative analysis (n=5–6). (H) Left: the representative blots of AMP-activated protein kinase, acetyl-coA carboxylase, mammalian target of rapamycin complex 1, S6 ribosomal protein and GAPDH were shown. Right: quantitative analysis (n=5–6). Results are expressed as mean±SEM; One-way ANOVA (non-repeated measures) (A–H). ACC indicates acetyl-coA carboxylase; AMPK, adenosine monophosphate-activated protein kinase; CD36, cluster of differentiation 36; mTOR, mammalian targe of rapamycin; S6, ribosomal protein S6; and TAC, transverse aortic constriction. *P<0.05 vs sham, †P<0.05 vs transverse aortic constriction.

Article Snippet: Antibodies against phospho- ACC (Cell signal, 3661), ACC (Cell signal, 3662), phospho- AMPK (Cell signal, 2535), AMPK (Cell signal, 5831), phosphomammalian target of rapamycin (mTOR) (Cell signal, 2971), mTOR (Cell signal, 2983), GAPDH (Cell signal, 2118), phospho- S6 ribosomal protein (Cell signal, 2217), S6 ribosomal protein (Cell signal, 2217), CD36 (cluster of differentiation 36) (Novus, NB400- 104), PPARα (peroxisome proliferator- activated receptoralpha) (Novus, NBP1- 04676), SERCA2 (Cell signal, 9580), phospho- CaMKII (Cell signal, 12716), phospholamban (Cell signal, 14562), and ryanodine receptor (Proteintech, 19765- 1- AP) were used as primary antibodies.

Techniques: Phospho-proteomics, Isolation

Journal: Journal of the American Heart Association

Article Title: Direct Cardiac Actions of the Sodium Glucose Co‐Transporter 2 Inhibitor Empagliflozin Improve Myocardial Oxidative Phosphorylation and Attenuate Pressure‐Overload Heart Failure

doi: 10.1161/jaha.120.018298

Figure Lengend Snippet: Figure 7. Empagliflozin binds glucose transporters and improves cardiac metabolism in heart failure. In failing hearts, empagliflozin binding to glucose transporters (eg, GLUT1, GLUT4) reduces the relative excessive glycolysis, increases CD36 (cluster of differentiation 36) expression to restore fatty acid uptake, and improves oxidative phosphorylation. Furthermore, decreased glucose uptake may lead to impaired pentose phosphate pathway, which can activate AMP-activated protein kinase and inhibit mammalian target of rapamycin complex 1 to attenuate cardiac hypertrophy. AMPK indicates adenosine monophosphate- activated protein kinase; CD36, cluster of differentiation 36; GLUTs, glucose transporters; mTORC1, mammalian target of rapamycin complex 1; OXPHOS, oxidative phosphorylation; and PPP, the pentose phosphate pathway.

Article Snippet: Antibodies against phospho- ACC (Cell signal, 3661), ACC (Cell signal, 3662), phospho- AMPK (Cell signal, 2535), AMPK (Cell signal, 5831), phosphomammalian target of rapamycin (mTOR) (Cell signal, 2971), mTOR (Cell signal, 2983), GAPDH (Cell signal, 2118), phospho- S6 ribosomal protein (Cell signal, 2217), S6 ribosomal protein (Cell signal, 2217), CD36 (cluster of differentiation 36) (Novus, NB400- 104), PPARα (peroxisome proliferator- activated receptoralpha) (Novus, NBP1- 04676), SERCA2 (Cell signal, 9580), phospho- CaMKII (Cell signal, 12716), phospholamban (Cell signal, 14562), and ryanodine receptor (Proteintech, 19765- 1- AP) were used as primary antibodies.

Techniques: Binding Assay, Expressing, Phospho-proteomics

Journal: Diagnostics

Article Title: Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

doi: 10.3390/diagnostics10040246

Figure Lengend Snippet: Comparison of mRNA expression in paranasal sinus mucosa from the controls, and CRSsNP and CRSwNP patients as detected by RT-PCR. ( a ) scavenger receptor class B type 1 (SR-B1) and ( b ) lectin-like oxidized LDL receptor-1 (LOX-1) mRNA levels were quantitatively normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA levels. Center lines: median values. Boxes: interquartile ranges. Error bars: overall ranges. NS: not significant.

Article Snippet: The primary antibodies used were antihuman SR-B1 rabbit polyclonal antibody (#5193; ProSci, Poway, CA, USA), antihuman LOX-1 rabbit polyclonal antibody (#11837-1-AP; Proteintech, Rosemont, IL, USA), and antihuman CD68 mouse monoclonal antibody (#M0814; Dako, Glostrup, Denmark).

Techniques: Comparison, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Diagnostics

Article Title: Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

doi: 10.3390/diagnostics10040246

Figure Lengend Snippet: Correlation between the severity of computed tomography (CT) findings and mRNA expression levels for ( a ) SR-B1 and ( b ) LOX-1 in sinus mucosa.

Article Snippet: The primary antibodies used were antihuman SR-B1 rabbit polyclonal antibody (#5193; ProSci, Poway, CA, USA), antihuman LOX-1 rabbit polyclonal antibody (#11837-1-AP; Proteintech, Rosemont, IL, USA), and antihuman CD68 mouse monoclonal antibody (#M0814; Dako, Glostrup, Denmark).

Techniques: Computed Tomography, Expressing

Journal: Diagnostics

Article Title: Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

doi: 10.3390/diagnostics10040246

Figure Lengend Snippet: Representative immunohistological images showing SR-B1 ( a , b ), LOX-1 ( c , d ), and CD68 ( e , f ) expression in ethmoid sinus mucosa sampled from a CRSwNP patient. Vascular endothelial cells (arrowheads) are stained positively both for SR-B1 and LOX-1. In contrast, numerous submucosal inflammatory cells show intense positive staining for LOX-1 compared to that for SR-B1. Scale bar: 20 μm.

Article Snippet: The primary antibodies used were antihuman SR-B1 rabbit polyclonal antibody (#5193; ProSci, Poway, CA, USA), antihuman LOX-1 rabbit polyclonal antibody (#11837-1-AP; Proteintech, Rosemont, IL, USA), and antihuman CD68 mouse monoclonal antibody (#M0814; Dako, Glostrup, Denmark).

Techniques: Expressing, Staining

Journal: Foods

Article Title: The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion

doi: 10.3390/foods10112852

Figure Lengend Snippet: Antibodies used for Western blot.

Article Snippet: Rabbit polyclonal Anti-Scavenging Receptor SR-BI antibody , Abcam , ab106572.

Techniques: Western Blot